DNA-based Tests for Mycoplasma and Chlamydia

Did you know that VITA-TECH performs DNA-based diagnostic tests for Mycoplasma as well as Chlamydia? These tests are an excellent alternative to current culture techniques for Mycoplasma and antigen detection of Chlamydia by immunoassay (antigen detection by antibody). DNA-testing uses the polymerase chain reaction (PCR) amplification to detect DNA sequences specific to the organism of interest. DNA-testing is highly specific and sensitive enough to detect a few organisms, even dead ones. This is a great advantage over standard culture of fragile pathogens such as mycoplasmas which frequently die during transportation and storage of specimens. DNA-testing also offers speed; the average sample turnaround time is three days, compared to an average two week wait for Mycoplasma culture results. Culture is still of diagnostic value for the identification of specific Mycoplasma and Ureaplasma species.

While the immunoassay for Chlamydia is also a rapid test and does not require live organisms, it relies on the presence of significant amounts of Chlamydia organisms in the sample and is therefore less sensitive than DNA-testing. Specificity is also a problem with the Chlamydia immunoassay, as false-positives can occur due to cross-reactions with other bacteria.

Samples for DNA-testing can be fluids, exudates, feces, and tissues, or swab samples collected with a plain sterile rayon swab and transported in a red-top Vacutainer tube. Transport medium and refrigeration are not necessary as DNA is very stable. For further information about the use of DNA-testing in disease diagnosis please call our DNA Department.